Composition chimique et propriétés antibactériennes des huiles essentielles d’Ocimum basilicum et d’Hyptis suaveolens (L.) Poit récoltés dans la région de Dakar au Sénégal

Les huiles essentielles des feuilles d’Ocimum basilicumet d’Hyptis suaveolens, récoltées dans la région de Dakar au Sénégal ont été extraites par entraînement à la vapeur et analysées en CPG et CPG-SM. Les monoterpènes oxygénés dont l’estragol (38,78 %), le linalol (19,45 %) et le méthyl-eugénol (9,98 %) sont majoritaires dans l’huile essentielle d’O. basilicum. Ils sont suivis d’un sesquiterpène hydrocarboné: le bergamotène (8,48 %). Par contre, l’huile essentielle de H. suaveolensest essentiellement constituée de composés hydrocarbonés: le β-caryophyllène (16,63 %), le sabinène (11,30 %), le terpinolène (8,58 %), le limonène (8,45 %) et le bergamotène (5,26 %). Les propriétés antimicrobiennes des huiles essentielles de ces plantes ont été testées in vitro sur cinq souches bactériennes (Staphylococcus aureus, Bacillus sp.,Salmonella sp., Escherichia coli, Streptococcus sp.). Une activité inhibitrice des huiles sur les souches étudiées a été observée. Toutefois, celle d’O.basilicums’est révélée plus active, particulièrement contre Bacillus sp., Salmonella sp., et Escherichia coli.Mots-clés: Ocimum basilicum, Hyptis suaveolens, huiles essentielles, composition chimique, propriétés antimicrobiennes. Chemical composition and antimicrobial properties of the essential oils of Ocimum basilicum andHyptis suaveolensharvested from Dakar region in SenegalEssential oils of leaves from Ocimum basilicum and Hyptis suaveolens collected in the region of Dakar in Senegal have been extracted by steam distillation and analyzed by GC and GC-MS. The oxygenated monoterpenes which estragol (38.78%), linalool (19.45%) and methyl-eugenol (9.98%) constitute the major portion of the essential oils of O. basilicum followed by bergamotene (8.48%) which is a sesquiterpene hydrocarbon. Principal compounds of H.suaveolens essential oilsare: β-caryophyllene (16.63%), sabinene (11.30%), terpinolene (8.58%), limonene (8.45%) and bergamotene (5.26%).The antimicrobial properties of essential oils of these plants were tested in vitro against five bacterial strains (Staphylococcus aureus, Bacillussp, Salmonella sp., Escherichia coli, Streptococcus sp.). The inhibitory activity of the oils on the strains studied was observed. However, the essential oil of O. basilicum was more active, especially against Bacillus sp., Salmonella sp. and Escherichia coli.Keywords: Ocimum basilicum, Hyptis suaveolens, essential oils, chemical composition, antimicrobial properties

Tiques et hémoparasitoses du bétail au Sénégal. III. La zone Nord-soudanienne

Les auteurs rapportent les résultats d'une étude sur les tiques et les hémoparasitoses des bovins, des ovins et des caprins de la zone nord-soudanienne. Un détiquage systématique de 40 bovins, 40 moutons et 40 chèvres est effectué pendant 15 mois dans le but de déterminer la dynamique des populations et de préciser les sites préférentiels de fixation des différentes espèces. Les espèces suivantes sont récoltées sur ces animaux : Hyalomma marginatum rufipes, H. truncatum, Rhipicephalus lunulatus, Rh. e evertsi, Rh. sulcatus, Rh. senegalensis, Boophilus decoloratus. Des études sont menées simultanément sur les hémoparasitoses, par réalisation de frottis de sang et de splénectomies. Chez les bovins, sont mis en évidence : Anaplasma marginale, Ehrlichia bovis, Theileria mutans, Th. velifera, Trypanosoma congolense, T. brucei et des microfilaires de Setaria labiatopapillosa. Babesia bigemina a été observée après splénectomie. Les infections décelées chez les petits ruminants sont occasionnées par A. ovis, Th. ovis et T. vivax. Les valeurs de l'hématocrite d'animaux apparemment sains sont étudiées de même que les variations saisonnières de ce paramètre hématologique

The roles of static stability and tropical – extratropical interactions in the summer interannual variability of the North Atlantic sector

Summer seasonal forecast skill in the North Atlantic sector is lower than winter skill. To identify potential controls on predictability, the sensitivity of North Atlantic baroclinicity to atmospheric drivers is quantified. Using ERA-INTERIM reanalysis data, North Atlantic storm-track baroclinicity is shown to be less sensitive to meridional temperature-gradient variability in summer. Static stability shapes the sector’s interannual variability by modulating the sensitivity of baroclinicity to variations in meridional temperature gradients and tropopause height and by modifying the baroclinicity itself. High static stability anomalies at upper levels result in more zonal extratropical cyclone tracks and higher eddy kinetic energy over the British Isles in the summertime. These static stability anomalies are not strongly related to the summer NAO; but they are correlated with the suppression of convection over the tropical Atlantic and with a poleward-shifted subtropical jet. These results suggest a non-local driver of North Atlantic variability. Furthermore, they imply that improved representations of convection over the south-eastern part of North America and the tropical Atlantic might improve summer seasonal forecast skill

The complete genome sequence of the phytopathogenic fungus Sclerotinia sclerotiorum reveals insights into the genome architecture of broad host range pathogens

Sclerotinia sclerotiorum is a phytopathogenic fungus with over 400 hosts including numerous economically important cultivated species. This contrasts many economically destructive pathogens that only exhibit a single or very few hosts. Many plant pathogens exhibit a “two-speed” genome. So described because their genomes contain alternating gene rich, repeat sparse and gene poor, repeat-rich regions. In fungi, the repeat-rich regions may be subjected to a process termed repeat-induced point mutation (RIP). Both repeat activity and RIP are thought to play a significant role in evolution of secreted virulence proteins, termed effectors. We present a complete genome sequence of S. sclerotiorum generated using Single Molecule Real-Time Sequencing technology with highly accurate annotations produced using an extensive RNA sequencing data set. We identified 70 effector candidates and have highlighted their in planta expression profiles. Furthermore, we characterized the genome architecture of S. sclerotiorum in comparison to plant pathogens that exhibit “two-speed” genomes. We show that there is a significant association between positions of secreted proteins and regions with a high RIP index in S. sclerotiorum but we did not detect a correlation between secreted protein proportion and GC content. Neither did we detect a negative correlation between CDS content and secreted protein proportion across the S. sclerotiorum genome. We conclude that S. sclerotiorum exhibits subtle signatures of enhanced mutation of secreted proteins in specific genomic compartments as a result of transposition and RIP activity. However, these signatures are not observable at the whole-genome scale

Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase

Pattern recognition receptors (PRRs) play a key role in plant and animal innate immunity. PRR binding of their cognate ligand triggers a signaling network and activates an immune response. Activation of PRR signaling must be controlled prior to ligand binding to prevent spurious signaling and immune activation. Flagellin perception in Arabidopsis through FLAGELLIN‐SENSITIVE 2 (FLS2) induces the activation of mitogen‐activated protein kinases (MAPKs) and immunity. However, the precise molecular mechanism that connects activated FLS2 to downstream MAPK cascades remains unknown. Here, we report the identification of a differentially phosphorylated MAP kinase kinase kinase that also interacts with FLS2. Using targeted proteomics and functional analysis, we show that MKKK7 negatively regulates flagellin‐triggered signaling and basal immunity and this requires phosphorylation of MKKK7 on specific serine residues. MKKK7 attenuates MPK6 activity and defense gene expression. Moreover, MKKK7 suppresses the reactive oxygen species burst downstream of FLS2, suggesting that MKKK7‐mediated attenuation of FLS2 signaling occurs through direct modulation of the FLS2 complex

Cellular development and evolution of the mammalian cerebellum

\ua9 2023, The Author(s).The expansion of the neocortex, a hallmark of mammalian evolution 1,2, was accompanied by an increase in cerebellar neuron numbers 3. However, little is known about the evolution of the cellular programmes underlying the development of the cerebellum in mammals. In this study we generated single-nucleus RNA-sequencing data for around 400,000 cells to trace the development of the cerebellum from early neurogenesis to adulthood in human, mouse and the marsupial opossum. We established a consensus classification of the cellular diversity in the developing mammalian cerebellum and validated it by spatial mapping in the fetal human cerebellum. Our cross-species analyses revealed largely conserved developmental dynamics of cell-type generation, except for Purkinje cells, for which we observed an expansion of early-born subtypes in the human lineage. Global transcriptome profiles, conserved cell-state markers and gene-expression trajectories across neuronal differentiation show that cerebellar cell-type-defining programmes have been overall preserved for at least 160 million years. However, we also identified many orthologous genes that gained or lost expression in cerebellar neural cell types in one of the species or evolved new expression trajectories during neuronal differentiation, indicating widespread gene repurposing at the cell-type level. In sum, our study unveils shared and lineage-specific gene-expression programmes governing the development of cerebellar cells and expands our understanding of mammalian brain evolution

A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria

Artemisinins are the cornerstone of anti-malarial drugs. Emergence and spread of resistance to them raises risk of wiping out recent gains achieved in reducing worldwide malaria burden and threatens future malaria control and elimination on a global level. Genome-wide association studies (GWAS) have revealed parasite genetic loci associated with artemisinin resistance. However, there is no consensus on biochemical targets of artemisinin. Whether and how these targets interact with genes identified by GWAS, remains unknown. Here we provide biochemical and cellular evidence that artemisinins are potent inhibitors of Plasmodium falciparum phosphatidylinositol-3-kinase (PfPI3K), revealing an unexpected mechanism of action. In resistant clinical strains, increased PfPI3K was associated with the C580Y mutation in P. falciparum Kelch13 (PfKelch13), a primary marker of artemisinin resistance. Polyubiquitination of PfPI3K and its binding to PfKelch13 were reduced by the PfKelch13 mutation, which limited proteolysis of PfPI3K and thus increased levels of the kinase, as well as its lipid product phosphatidylinositol-3-phosphate (PI3P). We find PI3P levels to be predictive of artemisinin resistance in both clinical and engineered laboratory parasites as well as across non-isogenic strains. Elevated PI3P induced artemisinin resistance in absence of PfKelch13 mutations, but remained responsive to regulation by PfKelch13. Evidence is presented for PI3P-dependent signalling in which transgenic expression of an additional kinase confers resistance. Together these data present PI3P as the key mediator of artemisinin resistance and the sole PfPI3K as an important target for malaria elimination